p akt2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc p akt2
P Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+akt2/Phospho-Akt2+(Ser474)+Rabbit+mAb/pm41714926-106-23-26
Average 95 stars, based on 187 article reviews

p akt2 - by Bioz Stars, 2026-09

95/100 stars

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Article Title: Investigation of the function of the PI3-Kinase / AKT signaling pathway for leukemogenesis and therapy of acute childhood lymphoblastic leukemia (ALL).
Article Snippet: Acute lymphoblastic leukemia is the most common cause of cancer-related death in children and, especially for patients in a high-risk group, still represents a poor prognosis.. The PI3K/AKT/mTOR signaling pathway has been identified as a frequently constitutively activated switching point in the disease of ALL.. Despite the knowledge of the therapeutic importance of the signaling pathway, the results of clinically effective treatment strategies have so far been extremely sobering.

Incubation:

Article Title: Mitochondrial genome modulates myocardial Akt/Glut/HK salvage pathway in spontaneously hypertensive rats adapted to chronic hypoxia.
Article Snippet: .. Fixed cryosections were incubated with the following primary monoclonal antibodies: mouse anti-GLUT1 and mouse anti-GLUT4 (Abcam), rabbit anti-HK2 (Abcam) and anti-OXPHOS antibody cocktail (Abcam) as a marker of the mitochondrial compartment, mouse antiSERCA2 (Sigma), and rabbit anti-AKT2 (Cell Signaling). .. Secondary goat anti-mouse IgG conjugated with Alexa Fluor 647 (Invitrogen), donkey anti-rabbit IgG conjugated with Alexa Fluor 488 (Invitrogen, Molecular Probes) or anti-rabbit IgG Cy5 was then used.

Article Title: Diabetic phenotype in mouse and humans reduces the number of microglia around β-amyloid plaques
Article Snippet: Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1x Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 h at RT. .. Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at + 4 °C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474, 1:1000, #8599, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt (Thr308/309/305, 1:1000, #13038, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt1 (1:1000, #75692, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt2 (1:1000, #3063, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, #9272, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-GSK3β (S9, 1:1000, #9336, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-GSK3β (1:1000, #9315, Cell Signaling Technology, Danvers, MA, USA), custom-made mouse anti-phospho-Tau (B6, 1:1000) [ ], mouse anti-4R-Tau (RD4, 1:1000, 05–804, Millipore), mouse anti-SQSTM1/p62 (1:1000, #5114, Cell Signaling Technology, Danvers, MA, USA), mouse anti-LC3 (1:1000, ab51520, Abcam, Cambridge, UK), mouse anti-Caspase-3 (1:1000, #9662, Cell Signaling Technology, Danvers, MA, USA), mouse anti-phospho-ERK (1:500, sc-7383, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-ERK2 (1:500, sc-154, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-p85α (1:1000, ABIN1098111, Antibodies-online GmbH, Aachen, Germany), rabbit anti-phospho-SYK (1:1000, MA5–14918, Invitrogen, Waltham, MA, USA), rabbit anti-SYK (1:1000, #13198, Cell Signaling Technology, Danvers, MA, USA), mouse anti-β-actin (1:1000, ab8226, Abcam, Cambridge, UK) and mouse anti-GAPDH (1:15000, ab8245, Abcam). .. Blots were subsequently probed with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies, either sheep anti-mouse-HRP (1:5000, NA931V, GE Healthcare, Chicago, IL, USA) or donkey anti-rabbit-HRP (1:5000, NA934V, GE Healthcare, Chicago, IL, USA) diluted in 1x TBST and incubated for 1 h at room temperature.

Article Title: Diabetic phenotype in mouse and humans with β-amyloid pathology reduces the number of microglia around β-amyloid plaques
Article Snippet: Unspecific antibody binding was prevented by incubating the blots in blocking solution containing 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in 1x Tris-buffered saline with 0.1 % Tween 20 (TBST) for 1 h at RT. .. Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at +4°C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474, 1:1000, #8599, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt (Thr308/309/305, 1:1000, #13038, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt1 (1:1000, #75692, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt2 (1:1000, #3063, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, #9272, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-GSK3β (S9, 1:1000, #9336, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-GSK3β (1:1000, #9315, Cell Signaling Technology, Danvers, MA, USA), custom-made mouse anti-phospho-Tau (B6, 1:1000) [ ], mouse anti-4R-Tau (RD4, 1:1000, 05-804, Millipore), mouse anti-SQSTM1/p62 (1:1000, #5114, Cell Signaling Technology, Danvers, MA, USA), mouse anti-LC3 (1:1000, ab51520, Abcam, Cambridge, UK), mouse anti-Caspase-3 (1:1000, #9662, Cell Signaling Technology, Danvers, MA, USA), mouse anti-phospho-ERK (1:500, sc-7383, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-ERK2 (1:500, sc-154, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-p85α (1:1000, ABIN1098111, Antibodies-online GmbH, Aachen, Germany), rabbit anti-phospho-SYK (1:1000, MA5-14918, Invitrogen, Waltham, MA, USA), rabbit anti-SYK (1:1000, #13198, Cell Signaling Technology, Danvers, MA, USA), mouse anti-β-actin (1:1000, ab8226, Abcam, Cambridge, UK) and mouse anti-GAPDH (1:15000, ab8245, Abcam). .. Blots were subsequently probed with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies, either sheep anti-mouse-HRP (1:5000, NA931V, GE Healthcare, Chicago, IL, USA) or donkey anti-rabbit-HRP (1:5000, NA934V, GE Healthcare, Chicago, IL, USA) diluted in 1x TBST and incubated for 1 h at room temperature.

Article Title: Intranasal insulin activates Akt2 signaling pathway in the hippocampus of wild-type but not in APP/PS1 Alzheimer model mice.
Article Snippet: .. Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at þ4 C: rabbit anti- phospho-Akt1 (Ser473, 1:1000, #9018; Cell Signaling Technology), rabbit anti-phospho-Akt2 (Ser474, 1:1000, #8599; Cell Signaling Technology), rabbit anti-phospho-Akt (Thr308/309/305, 1:1000, #13038; Cell Signaling Technology), rabbit anti-Akt1 (1:1000, #75692; Cell Signaling Technology), rabbit anti-Akt2 (1:1000, #3063; Cell Signaling Technology), rabbit anti-Akt (1:1000, #9272; Cell Signaling Technology), rabbit anti-phospho-GSK3b (Ser9, 1:1000, #9336; Cell Signaling Technology), rabbit anti-GSK3b (1:1000, #9315; Cell Signaling Technology), custom-made mouse anti-phospho-tau detecting the Ser202, Thr205, and Ser208 residues (B6, 1:1000), mouse anti-4R-Tau (RD4, 1:1000, 05-804; Millipore), mouse anti-SQSTM1/p62 (1:1000, #5114; Cell Signaling Technology), mouse anti-LC3 (1:1000, ab51520), and mouse antiGAPDH (1:15,000, ab8245; Abcam). .. Blots were subsequently probed with the appropriate horseradish peroxidase (HRP)econjugated secondary antibodies, either sheep anti-mouse-HRP (1:5000, NA931V; GE Healthcare) or donkey anti-rabbit-HRP (1:5000, NA934V; GE Healthcare) diluted in 1x TBST and incubated for 1 hour at room temperature.

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Article Title: Integrin and autocrine IGF2 pathways control fasting insulin secretion in β-cells
Article Snippet: Antibodies and reagents by guest on Septem ber 19, 2020 http://w w w .jbc.org/ D ow nloaded from The following antibodies were used: Cell Signaling Technology: rabbit anti-Akt1 (#2938), rabbit anti-Akt2 (#3063), rabbit anti-ERK1/2 (#9102), rabbit anti-p(Y118) paxillin (#2541), rabbit anti-pS473 Akt (#9271), rabbit anti-Akt (#9272), rabbit anti-Src (#2108).

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